Summary
Type: SPE
Application #: 305270
Catalog#: 730003 - SPE Cartridges, Chromabond, C18, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning: 2 ml methanol, then 2 ml 0.1 M acetic acid (flow rate: 2 ml/min); Do not let run the column dry!
Sample aspiration: The sample were rinsed through the sorbent bed (flow rate: 1 ml/min).
Washing: The SPE column were washed with 1 ml 0.1 M acetic acid and acetonitrile/water (70:30, v/v); (flow rate: 1 ml/min).
Drying: Reduced pressure was applied for 10 min.
Elution: With 1.5 ml acetonitrile (flow rate: 1 ml/min).
Evaporation: The extracts were evaporated to dryness at 60 °C under a stream of nitrogen.
Authors: Jung, J.; Auwaerter, V.; Herzog, G.; Mahler, H.; Kempf, J.; Weinmann, W.
Source: Inst. f. Rechtsmedizin, Freiburg